Effects of natural products (potato, banana, tomato and coconut water) and the period of exposure to 10.0㎎/ℓ BAP supplemented to Murashige-Skoog medium, on the proliferation and organogenesis of several temperate cymbidium rhizomes obtained from asymbiotic seed culture were measured in vitro.
Caulogenesis and rhizogenesis from rhizomes cultured on the NIS medium were influenced by species and rhizome sources which were derived from self- or cross-pollination between cymbidium species. In addition, rhizomes responded differently to the kinds and concentrations of natural products. Generally, proliferation of rhizomes was favored on the medium containing 3% banana homogenate, whereas shoot formation and subsequent growth promoted or, the medium supplemented with l0% coconut water except in case of C. forrestii `Garyung´ in which the growth was favored on the medium containing 20% banana homogenate.
Caulogenesis from rhizome was also affected by the period of exposure to the media with l0.0㎎/ℓ BAP. Favorable shoot formation and subsequent growth from cultured rhizome were achieved when rhizomes were exposed to10.0㎎/ℓ BAP-containing medium for 10 or 20 day s and then transferred to the medium with 0.5㎎/ℓ BAP.
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